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sheep anti secretagogin  (BioVendor Instruments)


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    BioVendor Instruments sheep anti secretagogin
    Sheep Anti Secretagogin, supplied by BioVendor Instruments, used in various techniques. Bioz Stars score: 93/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sheep+anti+secretagogin/Secretagogin+Human%2C+Sheep+Polyclonal+Antibody/pm41872139-256-18-21
    Average 93 stars, based on 20 article reviews
    sheep anti secretagogin - by Bioz Stars, 2026-10
    93/100 stars

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    other:

    Article Title: Dysfunction of α2δ4 leads to photoreceptor degeneration through disrupted synaptic mitochondria and calcium crosstalk
    Article Snippet: The following commercial antibodies were used: mouse anti-PKCα (AB11723, Abcam), rabbit anti-PKCα (P4334, Sigma-Aldrich), rabbit anti-secretagogin (RD181120100, BioVendor), sheep anti-secretagogin (RD184120100, BioVendor), rabbit anti-PSD95 (3450, Cell Signaling Technology), mouse anti-PSD95 (MA1-045, Invitrogen), guinea pig anti-vGlut1 (AB5905, Millipore), mouse anti-CTBP2 (612044, BD Biosciences), rabbit anti-cone arrestin (AB15282, Millipore), mouse anti-MtCo1 (AB14705, Abcam), rabbit anti-RYR-2 ( A34455 , antibodies.com), rabbit anti-IP3R1 (07-1213, Millipore), mouse anti-SERCA2 (MA3-910, Invitrogen), mouse anti-MCU (AMAB91189, Sigma Aldrich), mouse anti-CHOP (2895, Cell Signaling Technology), rabbit anti-phospho-elf2a (3398, Cell Signaling Technology), and rabbit anti-elf2a (5324, Cell Signaling Technology).

    Article Title: Dysfunction of α2δ4 leads to photoreceptor degeneration through disrupted synaptic mitochondria and calcium crosstalk.
    Article Snippet: The following commercial antibodies were used: mouse anti-PKCα (AB11723, Abcam), rabbit anti-PKCα (P4334, Sigma-Aldrich), rabbit anti-secretagogin (RD181120100, BioVendor), sheep anti-secretagogin (RD184120100, BioVendor), rabbit anti-PSD95 (3450, Cell Signaling Technology), mouse anti-PSD95 (MA1-045, Invitrogen), guinea pig anti-vGlut1 (AB5905, Millipore), mouse anti-CTBP2 (612044, BD Biosciences), rabbit anti-cone arrestin (AB15282, Millipore), mouse anti-MtCo1 (AB14705, Abcam), rabbit anti-RYR-2 (A34455, antibodies.com), rabbit anti-IP3R1 (07-1213, Millipore), mouse anti-SERCA2 (MA3-910, Invitrogen), mouse antiMCU (AMAB91189, Sigma Aldrich), mouse anti-CHOP (2895, Cell Signaling Technology), rabbit anti-phospho-elf2a (3398, Cell Signaling Technology), and rabbit anti-elf2a (5324, Cell Signaling Technology).

    Binding Assay:

    Article Title: Kainate Receptors Mediate Synaptic Input to Transient and Sustained OFF Visual Pathways in Primate Retina
    Article Snippet: .. The following primary antibodies and tissue culture supernatants were used; rabbit anti-recoverin (Millipore, #AB5585), rabbit or sheep anti-secretagogin (Biovendor R&D, #RD181120100, RD184120100), rabbit anti-glutamate transporter 1 (GLT-1, Tocris Bioscience, #2063), mouse anti-calbindin D28K (Sigma, #C9848), mouse anti-Islet-1 (Developmental Hybridoma Studies Bank, University of Iowa, #39.4D5), rabbit anti-calcium binding protein 5 (CaBP5, gift from Dr. F. Haeseleer), goat anti-GluK1 antibody (GluR5, Santa Cruz Biotechnology, SC-7616), goat anti-GluA3 (GluR3, Santa Cruz Biotechnology, sc-7612), rabbit anti-GluA4 (GluR4, Millipore, #AB1508), rabbit anti-neuropilin and tolloid-like 1 (Neto1, kindly provided by Dr Masahiko Watanabe, Hokkaido University ( Straub et al., 2011 ), mouse anti-RIBEYE (CtBP2, BD Biosciences, #612044), and mouse anti-PSD-95 (University of California at Davis/National Institutes of Health Neuromab #73–348, clone K28/74). ..

    Article Title: Kainate Receptors Mediate Synaptic Input to Transient and Sustained OFF Visual Pathways in Primate Retina
    Article Snippet: .. The following primary antibodies and tissue culture supernatants were used; rabbit anti-recoverin (Millipore, #AB5585), rabbit or sheep anti-secretagogin (Biovendor R&D, #RD181120100, RD184120100), rabbit anti-glutamate transporter 1 (GLT-1, Tocris Bioscience, #2063), mouse anti-calbindin D28K (Sigma, #C9848), mouse anti-Islet-1 (Developmental Hybridoma Studies Bank, University of Iowa, #39.4D5), rabbit anti-calcium binding protein 5 (CaBP5, gift from Dr. F. Haeseleer), goat anti-GluK1 antibody (GluR5, Santa Cruz Biotechnology, SC-7616), goat anti-GluA3 (GluR3, Santa Cruz Biotechnology, sc-7612), rabbit anti-GluA4 (GluR4, Millipore, #AB1508), rabbit anti-neuropilin and tolloid-like 1 (Neto1, kindly provided by Dr Masahiko Watanabe, Hokkaido University (Straub et al., 2011), mouse anti-RIBEYE (CtBP2, BD Biosciences, #612044), and mouse anti-PSD-95 (University of California at Davis/National Institutes of Health Neuromab #73–348, clone K28/74). ..

    Immunolabeling:

    Article Title: Heterogeneity of tyrosine hydroxylase expressing neurons in the main olfactory bulb of the mouse.
    Article Snippet: The structural features of dopamine (DA)-GABAergic neurons in the mouse main olfactory bulbs were examined, using both wild type and transgenic TH-GFP mice, with the combination of several methods; the immunocytochemistry, biotinylated dextran amine labeling, lucifer yellow injection in fixed slices, biocytin injection in live slice and the functional olfactory deprivation.. DA-GABAergic neurons were clustered in the glomerular layer (GL) but they also scattered in other layers.. DA-GABAergic juxtaglomerular neurons, were classified into 5 groups based on their structural features and named as follows: 1) Large periglomerular (LPG) cells with tuft-like glomerular dendritic branches and apparent axons extending to the distant glomeruli, which correspond to the " inhibitory juxtaglomerular association (IJGA) neurons" participating in the interglomerular association system. .. For the multiple-fluorescent immunolabeling sections were incubated in the blocking/dilution solution overnight, then incubated in a mixture of primary antibodies such as chicken anti-TH (Aves; 1:2,500), rabbit anti-ankyrin G (AnkG, Santa Cruz SC28561; 1:500) and sheep anti-secretagogin (SCGN, BioVendor, RD184120100; 1: 5,000) for 4–14 days at 20 ◦C. .. After rinsing in PBS the sections from wild type mice were incubated in a mixture of Alexa Fluor 488-conjugated donkey anti-chicken IgY (1:250; Jackson Immunoresearch, West Grove, PA, USA), indocarbocyanine (Cy3)-conjugated anti-rabbit IgG (1:1,000), indodicarbocyanine (Cy5)-conjugated anti-sheep IgG (1:250), and 4’,6-diamidino-2phenylindole (DAPI;1 g/ml; Dojindo, Kumamoto, Japan) for 12–24 h at 20 ◦C.

    Incubation:

    Article Title: Heterogeneity of tyrosine hydroxylase expressing neurons in the main olfactory bulb of the mouse.
    Article Snippet: The structural features of dopamine (DA)-GABAergic neurons in the mouse main olfactory bulbs were examined, using both wild type and transgenic TH-GFP mice, with the combination of several methods; the immunocytochemistry, biotinylated dextran amine labeling, lucifer yellow injection in fixed slices, biocytin injection in live slice and the functional olfactory deprivation.. DA-GABAergic neurons were clustered in the glomerular layer (GL) but they also scattered in other layers.. DA-GABAergic juxtaglomerular neurons, were classified into 5 groups based on their structural features and named as follows: 1) Large periglomerular (LPG) cells with tuft-like glomerular dendritic branches and apparent axons extending to the distant glomeruli, which correspond to the " inhibitory juxtaglomerular association (IJGA) neurons" participating in the interglomerular association system. .. For the multiple-fluorescent immunolabeling sections were incubated in the blocking/dilution solution overnight, then incubated in a mixture of primary antibodies such as chicken anti-TH (Aves; 1:2,500), rabbit anti-ankyrin G (AnkG, Santa Cruz SC28561; 1:500) and sheep anti-secretagogin (SCGN, BioVendor, RD184120100; 1: 5,000) for 4–14 days at 20 ◦C. .. After rinsing in PBS the sections from wild type mice were incubated in a mixture of Alexa Fluor 488-conjugated donkey anti-chicken IgY (1:250; Jackson Immunoresearch, West Grove, PA, USA), indocarbocyanine (Cy3)-conjugated anti-rabbit IgG (1:1,000), indodicarbocyanine (Cy5)-conjugated anti-sheep IgG (1:250), and 4’,6-diamidino-2phenylindole (DAPI;1 g/ml; Dojindo, Kumamoto, Japan) for 12–24 h at 20 ◦C.

    Blocking Assay:

    Article Title: Heterogeneity of tyrosine hydroxylase expressing neurons in the main olfactory bulb of the mouse.
    Article Snippet: The structural features of dopamine (DA)-GABAergic neurons in the mouse main olfactory bulbs were examined, using both wild type and transgenic TH-GFP mice, with the combination of several methods; the immunocytochemistry, biotinylated dextran amine labeling, lucifer yellow injection in fixed slices, biocytin injection in live slice and the functional olfactory deprivation.. DA-GABAergic neurons were clustered in the glomerular layer (GL) but they also scattered in other layers.. DA-GABAergic juxtaglomerular neurons, were classified into 5 groups based on their structural features and named as follows: 1) Large periglomerular (LPG) cells with tuft-like glomerular dendritic branches and apparent axons extending to the distant glomeruli, which correspond to the " inhibitory juxtaglomerular association (IJGA) neurons" participating in the interglomerular association system. .. For the multiple-fluorescent immunolabeling sections were incubated in the blocking/dilution solution overnight, then incubated in a mixture of primary antibodies such as chicken anti-TH (Aves; 1:2,500), rabbit anti-ankyrin G (AnkG, Santa Cruz SC28561; 1:500) and sheep anti-secretagogin (SCGN, BioVendor, RD184120100; 1: 5,000) for 4–14 days at 20 ◦C. .. After rinsing in PBS the sections from wild type mice were incubated in a mixture of Alexa Fluor 488-conjugated donkey anti-chicken IgY (1:250; Jackson Immunoresearch, West Grove, PA, USA), indocarbocyanine (Cy3)-conjugated anti-rabbit IgG (1:1,000), indodicarbocyanine (Cy5)-conjugated anti-sheep IgG (1:250), and 4’,6-diamidino-2phenylindole (DAPI;1 g/ml; Dojindo, Kumamoto, Japan) for 12–24 h at 20 ◦C.

    Oligomer Restriction:

    Article Title: Alterations in Kainate Receptor and TRPM1 Localization in Bipolar Cells after Retinal Photoreceptor Degeneration
    Article Snippet: .. The following primary antibodies were used at the specified dilutions; goat anti-GluK1 antibody (GluR5, Santa Cruz Biotechnology, SC-7616, 1:100), mouse anti-Islet-1 (Developmental Hybridoma Studies Bank, University of Iowa, #39.4D5, 1:250–500), rabbit anti-neuropilin and tolloid-like 1 (Neto1, kindly provided by Dr M. Watanabe, Hokkaido University, 1:320), rabbit anti-recoverin (Rec1, kindly provided by Dr. G. Adamus, Casey Eye Institute, OR, 1:200), rabbit or sheep anti-secretagogin (Biovendor R&D, #RD181120100, RD184120100, 1:2000–5000), rabbit anti-TRPM1 (Sigma, HPA014785, 1:4500), sheep anti-TRPM1 (kind gift from Dr K. Martemyanov, The Scripps Research Institute, FL, 1:500). .. Peanut Agglutinin Lectin (Alexa Fluor 488 conjugated) was obtained from Invitrogen/Life Technologies (L21409, 1:800).



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    Immunostaining of second order neurons. A , rod bipolar cells (RBCs) were stained with an anti-PKCα antibody in wild type and RX retinas. For cone bipolar cells (CBCs) <t>secretagogin</t> was used as marker. Arrowheads indicate elongated bipolar dendrites; (N = 4) and RX (N = 4); the scale bar represents 10 μm. B , bipolar cell dendrite occupancy in the ONL among CSNB2 variants. Cone bipolar cell dendrite occupancy in the ONL comparing wild type (0.06% ± 0.02, N = 4) and RX retinas (0.81% ± 0.32, N = 4), as well as previously published results for IT retinas (4.37% ± 0.49, N = 4; ). Rod bipolar cell dendrite occupancy: WT (0.13% ± 0.04, N = 4), RX (2.49% ± 0.45, N = 4) and IT retinae (3.16% ± 0.49, N = 6). C , double-labeling of horizontal cell (HC) processes with neurofilament 200 (NF200) and anti-calbindin in wild type and RX retinas. Arrows indicate axonal sprouting of horizontal cells; WT (N = 3) and RX (N = 5). The scale bar represents 10 μm. D , horizontal cell dendrite occupancy: WT (0.15% ± 0.13, N = 3), RX (1.14% ± 0.31, N = 5) and IT retinae (9.22% ± 0.83, N = 4). E , ratio of double-labeled horizontal cell dendrites (0.91 ± 0.05, RX: N = 5). Statistical analysis: One-way Anova with Tukey post hoc test; ns: p > 0.05; p value is indicated above; only significant p values are stated. CSNB2, congenital stationary night blindness type 2; ONL, outer nuclear layer.
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    Immunostaining of second order neurons. A , rod bipolar cells (RBCs) were stained with an anti-PKCα antibody in wild type and RX retinas. For cone bipolar cells (CBCs) <t>secretagogin</t> was used as marker. Arrowheads indicate elongated bipolar dendrites; (N = 4) and RX (N = 4); the scale bar represents 10 μm. B , bipolar cell dendrite occupancy in the ONL among CSNB2 variants. Cone bipolar cell dendrite occupancy in the ONL comparing wild type (0.06% ± 0.02, N = 4) and RX retinas (0.81% ± 0.32, N = 4), as well as previously published results for IT retinas (4.37% ± 0.49, N = 4; ). Rod bipolar cell dendrite occupancy: WT (0.13% ± 0.04, N = 4), RX (2.49% ± 0.45, N = 4) and IT retinae (3.16% ± 0.49, N = 6). C , double-labeling of horizontal cell (HC) processes with neurofilament 200 (NF200) and anti-calbindin in wild type and RX retinas. Arrows indicate axonal sprouting of horizontal cells; WT (N = 3) and RX (N = 5). The scale bar represents 10 μm. D , horizontal cell dendrite occupancy: WT (0.15% ± 0.13, N = 3), RX (1.14% ± 0.31, N = 5) and IT retinae (9.22% ± 0.83, N = 4). E , ratio of double-labeled horizontal cell dendrites (0.91 ± 0.05, RX: N = 5). Statistical analysis: One-way Anova with Tukey post hoc test; ns: p > 0.05; p value is indicated above; only significant p values are stated. CSNB2, congenital stationary night blindness type 2; ONL, outer nuclear layer.
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    Figure 3. Immunohistochemical analysis of retinal neuronal markers in Lyz2-p58IPK and Chx10- p58IPK cKO mice. Representative images of immunofluorescence staining for retinal neuronal markers for cone photoreceptors (cone arrestin), synapses between photoreceptors and bipolar cells (Ribeye), GABAergic amacrine <t>cells</t> <t>(GABA),</t> rod bipolar cells (PKC-α), and cone bipolar cells <t>(SCGN)</t> in retinal cryosections from WT, Lyz2-p58IPK cKO and Chx10-p58IPK cKO mice. Nuclei were stained with DAPI (blue). Scale bar = 50 µm.
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    Image Search Results


    Immunostaining of second order neurons. A , rod bipolar cells (RBCs) were stained with an anti-PKCα antibody in wild type and RX retinas. For cone bipolar cells (CBCs) secretagogin was used as marker. Arrowheads indicate elongated bipolar dendrites; (N = 4) and RX (N = 4); the scale bar represents 10 μm. B , bipolar cell dendrite occupancy in the ONL among CSNB2 variants. Cone bipolar cell dendrite occupancy in the ONL comparing wild type (0.06% ± 0.02, N = 4) and RX retinas (0.81% ± 0.32, N = 4), as well as previously published results for IT retinas (4.37% ± 0.49, N = 4; ). Rod bipolar cell dendrite occupancy: WT (0.13% ± 0.04, N = 4), RX (2.49% ± 0.45, N = 4) and IT retinae (3.16% ± 0.49, N = 6). C , double-labeling of horizontal cell (HC) processes with neurofilament 200 (NF200) and anti-calbindin in wild type and RX retinas. Arrows indicate axonal sprouting of horizontal cells; WT (N = 3) and RX (N = 5). The scale bar represents 10 μm. D , horizontal cell dendrite occupancy: WT (0.15% ± 0.13, N = 3), RX (1.14% ± 0.31, N = 5) and IT retinae (9.22% ± 0.83, N = 4). E , ratio of double-labeled horizontal cell dendrites (0.91 ± 0.05, RX: N = 5). Statistical analysis: One-way Anova with Tukey post hoc test; ns: p > 0.05; p value is indicated above; only significant p values are stated. CSNB2, congenital stationary night blindness type 2; ONL, outer nuclear layer.

    Journal: Molecular & Cellular Proteomics : MCP

    Article Title: Quantitative Proteomics Identifies Potential Molecular Adaptations in Mouse Models of Congenital Stationary Night Blindness Type 2

    doi: 10.1016/j.mcpro.2025.101462

    Figure Lengend Snippet: Immunostaining of second order neurons. A , rod bipolar cells (RBCs) were stained with an anti-PKCα antibody in wild type and RX retinas. For cone bipolar cells (CBCs) secretagogin was used as marker. Arrowheads indicate elongated bipolar dendrites; (N = 4) and RX (N = 4); the scale bar represents 10 μm. B , bipolar cell dendrite occupancy in the ONL among CSNB2 variants. Cone bipolar cell dendrite occupancy in the ONL comparing wild type (0.06% ± 0.02, N = 4) and RX retinas (0.81% ± 0.32, N = 4), as well as previously published results for IT retinas (4.37% ± 0.49, N = 4; ). Rod bipolar cell dendrite occupancy: WT (0.13% ± 0.04, N = 4), RX (2.49% ± 0.45, N = 4) and IT retinae (3.16% ± 0.49, N = 6). C , double-labeling of horizontal cell (HC) processes with neurofilament 200 (NF200) and anti-calbindin in wild type and RX retinas. Arrows indicate axonal sprouting of horizontal cells; WT (N = 3) and RX (N = 5). The scale bar represents 10 μm. D , horizontal cell dendrite occupancy: WT (0.15% ± 0.13, N = 3), RX (1.14% ± 0.31, N = 5) and IT retinae (9.22% ± 0.83, N = 4). E , ratio of double-labeled horizontal cell dendrites (0.91 ± 0.05, RX: N = 5). Statistical analysis: One-way Anova with Tukey post hoc test; ns: p > 0.05; p value is indicated above; only significant p values are stated. CSNB2, congenital stationary night blindness type 2; ONL, outer nuclear layer.

    Article Snippet: Secretagogin (sheep) , 1:1000 , BioVendor , RD184120100.

    Techniques: Immunostaining, Staining, Marker, Labeling

    Figure 3. Immunohistochemical analysis of retinal neuronal markers in Lyz2-p58IPK and Chx10- p58IPK cKO mice. Representative images of immunofluorescence staining for retinal neuronal markers for cone photoreceptors (cone arrestin), synapses between photoreceptors and bipolar cells (Ribeye), GABAergic amacrine cells (GABA), rod bipolar cells (PKC-α), and cone bipolar cells (SCGN) in retinal cryosections from WT, Lyz2-p58IPK cKO and Chx10-p58IPK cKO mice. Nuclei were stained with DAPI (blue). Scale bar = 50 µm.

    Journal: Cells

    Article Title: Neuronal p58 IPK Protects Retinal Ganglion Cells Independently of Macrophage/Microglia Activation in Ocular Hypertension.

    doi: 10.3390/cells12121558

    Figure Lengend Snippet: Figure 3. Immunohistochemical analysis of retinal neuronal markers in Lyz2-p58IPK and Chx10- p58IPK cKO mice. Representative images of immunofluorescence staining for retinal neuronal markers for cone photoreceptors (cone arrestin), synapses between photoreceptors and bipolar cells (Ribeye), GABAergic amacrine cells (GABA), rod bipolar cells (PKC-α), and cone bipolar cells (SCGN) in retinal cryosections from WT, Lyz2-p58IPK cKO and Chx10-p58IPK cKO mice. Nuclei were stained with DAPI (blue). Scale bar = 50 µm.

    Article Snippet: The primary antibodies used were: Ribeye (Synaptic Systems, Göttingen, Germany, 192003, 1:800); PKC-α (Santa Cruz, Dallas, TX, USA, sc-8393, 1:600); GABA (MilliporeSigma, Burlington, MA, USA, PC213L, 1:800); SCGN (BioVendor, Asheville, NC, USA, RD184120100); Iba1 (Wako, Richmond, VA, USA, 019-19741, 1:800); Cells 2023, 12, 1558 4 of 15 GFAP (Dako, 30334, 1:800); Cone Arrestin (MilliporeSigma, AB15282, 1:1000).

    Techniques: Immunohistochemical staining, Staining