Journal: Cells
Article Title: Neuronal p58 IPK Protects Retinal Ganglion Cells Independently of Macrophage/Microglia Activation in Ocular Hypertension.
doi: 10.3390/cells12121558
Figure Lengend Snippet: Figure 3. Immunohistochemical analysis of retinal neuronal markers in Lyz2-p58IPK and Chx10- p58IPK cKO mice. Representative images of immunofluorescence staining for retinal neuronal markers for cone photoreceptors (cone arrestin), synapses between photoreceptors and bipolar cells (Ribeye), GABAergic amacrine cells (GABA), rod bipolar cells (PKC-α), and cone bipolar cells (SCGN) in retinal cryosections from WT, Lyz2-p58IPK cKO and Chx10-p58IPK cKO mice. Nuclei were stained with DAPI (blue). Scale bar = 50 µm.
Article Snippet: The primary antibodies used were: Ribeye (Synaptic Systems, Göttingen, Germany, 192003, 1:800); PKC-α (Santa Cruz, Dallas, TX, USA, sc-8393, 1:600); GABA (MilliporeSigma, Burlington, MA, USA, PC213L, 1:800); SCGN (BioVendor, Asheville, NC, USA, RD184120100); Iba1 (Wako, Richmond, VA, USA, 019-19741, 1:800); Cells 2023, 12, 1558 4 of 15 GFAP (Dako, 30334, 1:800); Cone Arrestin (MilliporeSigma, AB15282, 1:1000).
Techniques: Immunohistochemical staining, Staining